rabbit anti human pdpn  (Proteintech)

Journal: Journal of Inflammation Research

Article Title: Mechanistic Study of CD90-Positive Synovial Fibroblasts in the Invasion and Recurrence of Pigmented Villonodular Synovitis

doi: 10.2147/JIR.S549953

Figure Lengend Snippet: Identification of 16 cell clusters with diverse annotations reveals a high abundance of PDPN(+) CD90(+) sublining fibroblasts in PVNS synovium based on scRNA-seq data. ( A ) Quality control of the scRNA-seq data in GSE155527 . ( B ) The UMAP algorithm showing 16 cell clusters after dimensionality reduction. ( C ) Heatmap showing expression of specific gene markers in each cell type. ( D ) The UMAP plot representation demonstrates the main cell types. ( E ) Immunofluorescence images of PDPN and CD90 in the synovium of the normal individuals and patients with OA and PVNS. Red: CD90, Green: PDPN, Blue: nucleus.

Article Snippet: The primary antibodies used were: mouse anti-human CD90 (1:100; Proteintech, China, 66766-1-Ig) and rabbit anti-human PDPN (1:100; Proteintech, China, 11629-1-AP) for immunofluorescence staining; mouse anti-human CD206 (1:100; Proteintech, China, 18704-1-AP) and rabbit anti-human CXCR2 (1:100; Proteintech, China, 20634-1-AP) for immunohistochemical staining.

Techniques: Control, Expressing, Immunofluorescence

Journal: Journal of Inflammation Research

Article Title: Mechanistic Study of CD90-Positive Synovial Fibroblasts in the Invasion and Recurrence of Pigmented Villonodular Synovitis

doi: 10.2147/JIR.S549953

Figure Lengend Snippet: PDPN(+) CD90(+) sublining fibroblasts in PVNS synovium exhibit enhanced migration and invasion capabilities. ( A ) Flow cytometric analysis of PDPN and CD90 double-positive primary FLSs populations in OA (up) and PVNS (down) synovium. ( B ) PDPN and CD90 immunofluorescence image of OA FLSs and PVNS FLSs. Red: CD90, Green: PDPN, Blue: nucleus. ( C ) Transwell migration (lower panel) and invasion (upper panel) assays of OA FLSs and PVNS FLSs. ( D ) Statistical analysis of transwell migration (lower panel) and invasion (upper panel) assays. ( E ) Wound-healing analysis of OA FLSs (up) and PVNS FLSs (down). ****P < 0.0001.

Article Snippet: The primary antibodies used were: mouse anti-human CD90 (1:100; Proteintech, China, 66766-1-Ig) and rabbit anti-human PDPN (1:100; Proteintech, China, 11629-1-AP) for immunofluorescence staining; mouse anti-human CD206 (1:100; Proteintech, China, 18704-1-AP) and rabbit anti-human CXCR2 (1:100; Proteintech, China, 20634-1-AP) for immunohistochemical staining.

Techniques: Migration, Immunofluorescence

Journal: Journal of Inflammation Research

Article Title: Mechanistic Study of CD90-Positive Synovial Fibroblasts in the Invasion and Recurrence of Pigmented Villonodular Synovitis

doi: 10.2147/JIR.S549953

Figure Lengend Snippet: PDPN(+) CD90(+) sublining fibroblasts express higher levels of pro-inflammatory cytokines and invasion related proteins. ( A ) Volcano plot of DEGs in PDPN(+) CD90(+) sublining fibroblasts between the OA and PVNS synovium. ( B ) KEGG pathway analysis of the DEGs. ( C ) UMAP plots showing the expression of inflammatory factors in OA and PVNS PDPN(+) CD90(+) sublining fibroblasts. ( D ) UMAP plots showing the expression of osteoclast-related proteins in OA and PVNS PDPN(+) CD90(+) sublining fibroblasts. ( E ) Representative images of hub protein expression detected by Western blot. ( F ) Statistical analysis of Western blot. All data are expressed as mean ± SD, n = 3. ns P > 0.05; ***P < 0.001; ****P < 0.0001.

Article Snippet: The primary antibodies used were: mouse anti-human CD90 (1:100; Proteintech, China, 66766-1-Ig) and rabbit anti-human PDPN (1:100; Proteintech, China, 11629-1-AP) for immunofluorescence staining; mouse anti-human CD206 (1:100; Proteintech, China, 18704-1-AP) and rabbit anti-human CXCR2 (1:100; Proteintech, China, 20634-1-AP) for immunohistochemical staining.

Techniques: Expressing, Western Blot

Journal: Acta dermato-venereologica

Article Title: Podoplanin Expression Correlates with Disease Progression in Mycosis Fungoides.

doi: 10.2340/00015555-2517

Figure Lengend Snippet: Fig. 2. Comparison of immunohistochemical expression of podoplanin (PDPN) with regard: to (A) disease presence and (B) clinical advancement. Comparison of immunohistochemical expression of vascular endothelial growth factor-C (VEGF-C) with regard to (C) disease presence and (D) clinical advancement. MF: mycosis fungoides; IHC: immunohistochemistry, early MF – stage IA–IIA; advanced MF – stage IIB–IVB. **p < 0.01, ****p < 0.0001, Mann–Whitney test.

Article Snippet: Subsequently, the membranes were incubated with rabbit anti-human VEGF-C antibody – 1:500 (AP2042c; Abgent, San Diego, CA, USA) and rabbit anti-human PDPN antibody – 1:750 (11629-1-AP; Proteintech Europe, Manchester, UK) for a night at 4°C.

Techniques: Comparison, Immunohistochemical staining, Expressing, Immunohistochemistry, MANN-WHITNEY

Journal: Acta dermato-venereologica

Article Title: Podoplanin Expression Correlates with Disease Progression in Mycosis Fungoides.

doi: 10.2340/00015555-2517

Figure Lengend Snippet: Fig. 3. Comparison of immunohistochemical expression of podoplanin (PDPN) with regard to: (A, B) skin involvement, (C) nodal involvement, and (D) staging. MF: mycosis fungoides; IHC: immunohistochemistry. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001; Mann–Whitney test.

Article Snippet: Subsequently, the membranes were incubated with rabbit anti-human VEGF-C antibody – 1:500 (AP2042c; Abgent, San Diego, CA, USA) and rabbit anti-human PDPN antibody – 1:750 (11629-1-AP; Proteintech Europe, Manchester, UK) for a night at 4°C.

Techniques: Comparison, Immunohistochemical staining, Expressing, Immunohistochemistry, MANN-WHITNEY

Journal: Acta dermato-venereologica

Article Title: Podoplanin Expression Correlates with Disease Progression in Mycosis Fungoides.

doi: 10.2340/00015555-2517

Figure Lengend Snippet: Fig. 4. Sample results of Western blot analysis of vascular endothelial growth factor C (VEGF-C) and podoplanin (PDPN) expression in mycosis fungoides (MF) and control cases.

Article Snippet: Subsequently, the membranes were incubated with rabbit anti-human VEGF-C antibody – 1:500 (AP2042c; Abgent, San Diego, CA, USA) and rabbit anti-human PDPN antibody – 1:750 (11629-1-AP; Proteintech Europe, Manchester, UK) for a night at 4°C.

Techniques: Western Blot, Expressing, Control

Journal: Acta dermato-venereologica

Article Title: Podoplanin Expression Correlates with Disease Progression in Mycosis Fungoides.

doi: 10.2340/00015555-2517

Figure Lengend Snippet: Fig. 5. Comparison of vascular endothelial growth factor C (VEGF-C) and podoplanin (PDPN) expression in Western blot (WB) analysis with regard to disease presence. MF: mycosis fungoides.

Article Snippet: Subsequently, the membranes were incubated with rabbit anti-human VEGF-C antibody – 1:500 (AP2042c; Abgent, San Diego, CA, USA) and rabbit anti-human PDPN antibody – 1:750 (11629-1-AP; Proteintech Europe, Manchester, UK) for a night at 4°C.

Techniques: Comparison, Expressing, Western Blot

Journal: Acta dermato-venereologica

Article Title: Podoplanin Expression Correlates with Disease Progression in Mycosis Fungoides.

doi: 10.2340/00015555-2517

Figure Lengend Snippet: Fig. 6. Spearman’s rank correlation plots presenting associations between expressions of podoplanin (PDPN) and vascular endothelial growth factor C (VEGF-C) analysed by immunohistochemistry (IHC) and Western blot (WB) technique: (A) PDPN WB vs. PDPN IHC (r = 0.75, p < 0.0001), (B) VEGF-C WB vs. VEGF-C IHC (r = 0.68, p = 0.0007), (C) VEGF-C IHC vs. PDPN IHC (r=0.70, p < 0.0001) and (D) VEGF-C WB vs. PDPN WB (r = 0.38, p = 0.083) in patients with mycosis fungoides.

Article Snippet: Subsequently, the membranes were incubated with rabbit anti-human VEGF-C antibody – 1:500 (AP2042c; Abgent, San Diego, CA, USA) and rabbit anti-human PDPN antibody – 1:750 (11629-1-AP; Proteintech Europe, Manchester, UK) for a night at 4°C.

Techniques: Immunohistochemistry, Western Blot, Immunohistochemistry-Resin

Journal: Acta dermato-venereologica

Article Title: Podoplanin Expression Correlates with Disease Progression in Mycosis Fungoides.

doi: 10.2340/00015555-2517

Figure Lengend Snippet: Fig. 7. Analysis of survival in the mycosis fungoides group according to (A) immunohistochemical expression of podoplanin (PDPN) and (B) vascular endothelial growth factor C (VEGF-C). **p < 0.001, Mantel-Cox test.

Article Snippet: Subsequently, the membranes were incubated with rabbit anti-human VEGF-C antibody – 1:500 (AP2042c; Abgent, San Diego, CA, USA) and rabbit anti-human PDPN antibody – 1:750 (11629-1-AP; Proteintech Europe, Manchester, UK) for a night at 4°C.

Techniques: Immunohistochemical staining, Expressing